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reporter gene  (10X Genomics)

 
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    10X Genomics reporter gene
    Reporter Gene, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reporter+gene/gene+reporter/pmc13122662-402-2-15
    Average 86 stars, based on 1 article reviews
    reporter gene - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Marker:

    Article Title: AMPK-p38 axis converts human pluripotent stem cells to naive state
    Article Snippet: For HISAT2 index build, Corresponded ensembl gtf file was filtered to retain protein-coded RNA, long non-coding RNA and T cell chain/immunoglobulin chain annotations according to 10X Genomics's method ( https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/advanced/references#mkgtf ). .. Addition of reporter gene (EGFP-IRES2-Puromycin-3'UTR) into reference genome and GTF files was performed according to 10X Genomics's method ( https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/using/tutorial_mr , “Add a Marker Gene to the FASTA and GTF” section). ..



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    Image Search Results


    A) Recombination strategy for tamoxifen (tmx; cyan)–mediated replacement of the HRas gene (grey, flanked by loxP sites) with HRasG12V (purple) in PlpCreERt2;FR-HRasG12V (pHRsG/+) mice. B) 2-month-old (2MO) mice treated with tmx are subjected to the complex wheel (CW) test, as well as histological and DTI-MRI analyses, 1-16 weeks later. C) Cell populations are analyzed in seven regions throughout the anterior-posterior (I-IV) and lateral “B” - central “C” axes of the corpus callosum (CC), unless otherwise disclosed. D) Immunostaining of coronal sections showing recombinant cells (GFP+, green arrowheads), OLs (GSTpi+, purple arrowheads), and GFP+GSTpi+ recombinant OLs (orange arrowheads) in the CC of WT and pHRsG/+ mice. E) Bar graph showing that the percentage (normalized to dapi) of recombinant cells in WT and pHRsG/+ mice is not significantly different (unpaired Student’s t test; males P= 0.14 and females P= 0.24). Genotype/sex color code and “n” per group in E, G : male WT (green) and pHRsG/+ (orange); female WT (black) and pHRsG/+ (red). F) Immunostaining picture indicating recombinant cells (green arrowheads), microglia (IBA1+, blue arrowheads), and OPCs (PDGFRa+, orange arrowheads) in the CC of WT and pHRsG/+ mice. G) The percentage of IBA+ cells in WT and pHRsG/+ mice (% of age/gender matched WTs) indicates significantly decreased # of microglia in male pHRsG/+ mice (unpaired Student’s t test; P= 0.047). Insets in D and F show the CC region in high magnification (dotted yellow square). Dapi was used to stain nuclei and normalize cell densities per arbitrary units (AU). Scale bar = 25μm. *P < 0.05.

    Journal: bioRxiv

    Article Title: Targeting Nitric Oxide Synthase 2 Reverses Learning Deficits in an Oligodendrocyte-Focused Model of Costello Syndrome

    doi: 10.64898/2026.06.01.729333

    Figure Lengend Snippet: A) Recombination strategy for tamoxifen (tmx; cyan)–mediated replacement of the HRas gene (grey, flanked by loxP sites) with HRasG12V (purple) in PlpCreERt2;FR-HRasG12V (pHRsG/+) mice. B) 2-month-old (2MO) mice treated with tmx are subjected to the complex wheel (CW) test, as well as histological and DTI-MRI analyses, 1-16 weeks later. C) Cell populations are analyzed in seven regions throughout the anterior-posterior (I-IV) and lateral “B” - central “C” axes of the corpus callosum (CC), unless otherwise disclosed. D) Immunostaining of coronal sections showing recombinant cells (GFP+, green arrowheads), OLs (GSTpi+, purple arrowheads), and GFP+GSTpi+ recombinant OLs (orange arrowheads) in the CC of WT and pHRsG/+ mice. E) Bar graph showing that the percentage (normalized to dapi) of recombinant cells in WT and pHRsG/+ mice is not significantly different (unpaired Student’s t test; males P= 0.14 and females P= 0.24). Genotype/sex color code and “n” per group in E, G : male WT (green) and pHRsG/+ (orange); female WT (black) and pHRsG/+ (red). F) Immunostaining picture indicating recombinant cells (green arrowheads), microglia (IBA1+, blue arrowheads), and OPCs (PDGFRa+, orange arrowheads) in the CC of WT and pHRsG/+ mice. G) The percentage of IBA+ cells in WT and pHRsG/+ mice (% of age/gender matched WTs) indicates significantly decreased # of microglia in male pHRsG/+ mice (unpaired Student’s t test; P= 0.047). Insets in D and F show the CC region in high magnification (dotted yellow square). Dapi was used to stain nuclei and normalize cell densities per arbitrary units (AU). Scale bar = 25μm. *P < 0.05.

    Article Snippet: Floating sections were processed for immunodetection using antibodies for the reporter gene GFP (Nacalai Tesque, Kyoto, Japan), and the cell-type markers GSTpi (MBL Ltd, Tokio, Japan), CC1 (Calbiochem, San Diego, CA, USA), NG2 (Millipore, Burlington, MA, USA), PDGFRa (Rn’D systems, Minneapolis, MN), GFAP (Dako, Santa Clara, CA, USA), IBA1 (Wako, Osaka, Japan), and NeuN (Millipore, St. Louis, MO).

    Techniques: Immunostaining, Recombinant, Staining